backbone pentr dmd donor vector Search Results


gateway pentr4 vector backbone  (New England Biolabs)
98
New England Biolabs gateway pentr4 vector backbone
Gateway Pentr4 Vector Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant dna reagent pentr d topo  (Thermo Fisher)
99
Thermo Fisher recombinant dna reagent pentr d topo
Recombinant Dna Reagent Pentr D Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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entry vector pentr  (Thermo Fisher)
90
Thermo Fisher entry vector pentr
Entry Vector Pentr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pentr dmd donor vector  (Addgene inc)
91
Addgene inc pentr dmd donor vector
Pentr Dmd Donor Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pentr dmd donor vector - by Bioz Stars, 2026-04
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pentr d topo vector backbone  (Thermo Fisher)
86
Thermo Fisher pentr d topo vector backbone
Pentr D Topo Vector Backbone, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pentr d topo vector backbone - by Bioz Stars, 2026-04
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pentr  (Thermo Fisher)
90
Thermo Fisher pentr
Pentr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pentr - by Bioz Stars, 2026-04
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pentr/d-topo  (Thermo Fisher)
90
Thermo Fisher pentr/d-topo
Pentr/D Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pentr/d-topo - by Bioz Stars, 2026-04
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mirneasy plasm/plasma kit  (Qiagen)
90
Qiagen mirneasy plasm/plasma kit
Mirneasy Plasm/Plasma Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector2 multilabel counter  (Vector Laboratories)
86
Vector Laboratories vector2 multilabel counter
Vector2 Multilabel Counter, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substrate vectora blue  (Vector Laboratories)
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Vector Laboratories substrate vectora blue
Substrate Vectora Blue, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3.1-nc  (Genechem)
90
Genechem pcdna3.1-nc
DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC <t>and</t> <t>pcDNA3.1-DNMT1</t> or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + <t>vector2</t>
Pcdna3.1 Nc, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e vectorh novaredtm  (Vector Laboratories)
86
Vector Laboratories e vectorh novaredtm
DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC <t>and</t> <t>pcDNA3.1-DNMT1</t> or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + <t>vector2</t>
E Vectorh Novaredtm, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC and pcDNA3.1-DNMT1 or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + vector2

Journal: Biological Research

Article Title: DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice

doi: 10.1186/s40659-021-00347-1

Figure Lengend Snippet: DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC and pcDNA3.1-DNMT1 or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + vector2

Article Snippet: The vector pcDNA3.1-DNMT1 overexpressed DNMT1, and the corresponding empty vector pcDNA3.1-NC (vector2) were constructed by GeneChem.

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Expressing, Flow Cytometry